Resistin inhibits rat insulinoma cell RINm5F proliferation / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 43-46, 2010.
Article
em Zh
| WPRIM
| ID: wpr-305111
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Resistin was thought to link the obesity to type 2 diabetes. This study aimed to investigate the effect of resistin on insulinoma cell proliferation.</p><p><b>METHODS</b>pcDNA3.1-resistin was transfected into rat insulinoma cells RINm5F. Cell proliferation was assessed by the MTT assay. The resistin and SOCS3 mRNA levels were assessed by RT-PCR. The total Akt level and the phosphorylation status were assessed by Western blot.</p><p><b>RESULTS</b>The over-expressed resistin inhibited the RINm5F cell proliferation (p<0.05). SOCS-3 expression was up-regulated by resistin over-expression (3.2 folds over the control; p<0.05). Akt phosphorylation was down-regulated by resistin over-expression (0.6 fold over the control; p<0.05).</p><p><b>CONCLUSIONS</b>Resistin impairs the rat insulinoma cell RINm5F proliferation. This might be attributed to a down-regulation of Akt level caused by increased SOCS-3 expression.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Neoplasias Pancreáticas
/
Patologia
/
Fosforilação
/
Fisiologia
/
Transfecção
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Resistina
/
Proteínas Proto-Oncogênicas c-akt
/
Proteínas Supressoras da Sinalização de Citocina
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2010
Tipo de documento:
Article