Establishment and identification of highly expressing and replicating hepatitis B virus genome transgenic mouse models / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 338-340, 2003.
Article
em Zh
| WPRIM
| ID: wpr-305949
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a highly expressing and replicating hepatitis B virus (HBV) genome transgenic mouse models for screening anti-HBV drugs and investigating the pathogenesis of hepatitis B.</p><p><b>METHODS</b>Elongated HBV genome as the investigated gene was transducted into the pronuclei of the fertilized eggs of mice by the technique of microinjection, then the eggs were transplanted into the oviducts of the pseudopregnant mice. All the newborn mice were screened and identified by PCR and Southern blot detecting genomic DNA in tail tissue, then the positive mice were examined plasma HBsAg, HBeAg by ELISA and plasma HBV DNA by Southern blot.</p><p><b>RESULTS</b>Among the 61 offsprings, 18 were positive for tail tissue HBV DNA examination, 7 of which were positive for replication and expression detection.</p><p><b>CONCLUSION</b>Transgenic mice with elongated HBV genome possess high efficiency of replication and expression, which can be used for further investigation.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fisiologia
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Virologia
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Replicação Viral
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DNA Viral
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Camundongos Transgênicos
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Vírus da Hepatite B
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Genoma Viral
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Modelos Animais de Doenças
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Replicação do DNA
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Genética
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Article