Regulatory effects of Wuzhuyutang (Evodiae prescription) and its consisting herbs on TPH2 promoter / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2261-2264, 2009.
Article
em Zh
| WPRIM
| ID: wpr-307605
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the active component of Wuzhuyutang (WZYT, Evodiae prescription) and investigate the regulatory effects of the components in WZYT on the TPH2 promoter, and to explore the possible molecular mechanism of WZYT on migraine.</p><p><b>METHOD</b>By transfecting a TPH2 promoter regulating Red Fluorescent Protein expressing plasmid into PC12 cell, the global fluorescence intensities and calculations of fluorescent cells after components treatment were statistically evaluated.</p><p><b>RESULT</b>Different regulatory effects of different components in WZYT with different concentrations on TPH2 promoter were observed.</p><p><b>CONCLUSION</b>TPH2 promoter drove Red Fluorescent Protein expressing cell line can be used as system screening components targeting TPH2 promoter activity. The possible mechanism of WZYT on migraine may due to its stimulating effects on TPH2 promoter, and promote the synthesis and release of 5-HT in cerebral.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Triptofano Hidroxilase
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Medicamentos de Ervas Chinesas
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Química
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Regiões Promotoras Genéticas
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Células PC12
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Evodia
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Tratamento Farmacológico
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Genética
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Metabolismo
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2009
Tipo de documento:
Article