Simultaneous determination of six flavonoids in Flos Chrysanthemi Indici by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2067-2070, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-307643
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of luteolin-7-O-glycoside, apigenin-7-O-glycoside, linarin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici simultaneously.</p><p><b>METHOD</b>The reversed phase HPLC system consisting of a C18 column (4.6 mm x 250 mm, 5 microm) and a mixture of acetonitrile and 0.05% phosphate acid with gradient elution as the mobile phase was adopted. The absorbance was monitored at 326 nm.</p><p><b>RESULT</b>The linear response range was 1.08-21.5 mg x L(-1) (r = 0.9990), 0.278-5.57 mg x L(-1) (r = 0.9994), 5.58-112 mg x L(-1) (r = 0.9999), 0.521-10.4 mg x L(-1) (r = 0.9995), 0.162-3.25 mg x L(-1) (r = 0.9999) and 0.288-5.76 mg x L(-1) (r = 0.9998), respectively (n = 6). The average recoveries (n = 9) of six flavonoids were 96.34%-104.0%. All of RSD of precision (n = 6) and repeatability (n = 6) were less than 1.2% and 2.6%.</p><p><b>CONCLUSION</b>The validation data demonstrated that the method was accurate and repeatable, and can be ase to measure the six flavonoids in Flos Chrysanthemi Indici.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Flavonoides
/
Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
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Chrysanthemum
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Cromatografia de Fase Reversa
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2009
Tipo de documento:
Artigo