Effects of Clostridium difficile toxin a on proliferation of K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 894-897, 2011.
Article
em Zh
| WPRIM
| ID: wpr-313870
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the effect of clostridium difficile toxin A (Tcd A) on proliferation of K562 cells and its mechanism. The proliferative activity of K562 cells exposed to Tcd A was tested by MTT assay; cell cycle distribution and mitochondrial membrane potential were analyzed by flow cytometry; the protein expression of cytochrome C and DNA fragmentation were observed by immunohistochemistry staining and agarose gel electrophoresis respectively. The results indicated that Tcd A inhibited proliferation of K562 cells in a time-and concentration-dependent manner. Cells were arrested at G(0)/G(1) phase. Peak of apoptosis appeared. The protein expression of cytochrome C increased as compared with control group (p < 0.05). Agarose gel electrophoresis of DNA from K562 treated with Tcd A revealed a "ladder" pattern. It is concluded that clostridium difficile toxin A can inhibit proliferation and induce apoptosis of K562 cells. The mechanism may be in relation to decrease of mitochondrial membrane potential and the release of cytochrome C from mitochondria matrix.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Toxinas Bacterianas
/
Apoptose
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Células K562
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Proliferação de Células
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Enterotoxinas
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Article