Inhibited proliferation and expression of human nasopharyngeal carcinoma line induced by celecoxib in vitro / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 809-812, 2006.
Article
em Zh
| WPRIM
| ID: wpr-315590
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of celecoxib on the cell proliferation and expression of vascular endothelial growth factor (VEGF) in human nasopharyngeal carcinoma line.</p><p><b>METHODS</b>3-[ 4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) test was used to investigate the cell proliferation. Flow cytometry (FCM) was used to analyze the cell cycle arrest. Immunocytochemistry technique was to observe the expression of VEGF.</p><p><b>RESULTS</b>Celecoxib inhibited the growth of nasopharyngeal carcinoma line, the cell number of G0/G1 phase increased from 62.13% to 91.35%, and the cell number of G2/M and S phase decreased from 21.59% to 3.56% and from 16.28% to 5.01%, respectively, cell cycle progression was arrested at G1/S phase. Celecoxib decreased the positive expression of VEGF in HNE-1 cells.</p><p><b>CONCLUSIONS</b>Celecoxib inhibited the proliferation of nasopharyngeal carcinoma significantly and the expression of VEGF.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
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Pirazóis
/
Sulfonamidas
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Neoplasias Nasofaríngeas
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Linhagem Celular Tumoral
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Fator A de Crescimento do Endotélio Vascular
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Proliferação de Células
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Celecoxib
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2006
Tipo de documento:
Article