Expressions of chemokine CXCL12 and its receptor CXCR4 in human sperm / 中华男科学杂志
National Journal of Andrology
; (12): 225-228, 2015.
Article
em Zh
| WPRIM
| ID: wpr-319515
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression patterns of the chemokine CXCL12 and its receptor CXCR4 in human sperm.</p><p><b>METHODS</b>We collected semen samples from 10 fertile men, performed density gradient centrifugation, and then determined the expressions of both CXCL12 and CXCR4 in the sperm by RT-PCR and immunofluorescence staining.</p><p><b>RESULTS</b>RT-PCR revealed the mRNA expressions of CXCL12 (0.641 +/- 0.180) and CXCR4 (0.464 +/- 0.100) in the sperm. However, only CXCR4 rather than CXCL12 was expressed at the protein level, and the positive staining for CXCR4 was observed mainly in the posterior part of the acrosome.</p><p><b>CONCLUSION</b>CXCL12 and CXCR4 are involved as important molecules in regulating the function of human sperm.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Espermatozoides
/
Acrossomo
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Transdução de Sinais
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Centrifugação com Gradiente de Concentração
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Receptores CXCR4
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Quimiocina CXCL12
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Metabolismo
Limite:
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2015
Tipo de documento:
Article