Evaluation of genetic diversity and population structure of Bletilla striata based on SRAP markers / 药学学报
Acta Pharmaceutica Sinica
; (12): 147-152, 2016.
Article
em Zh
| WPRIM
| ID: wpr-320002
Biblioteca responsável:
WPRO
ABSTRACT
Bletilla striata has been used as traditional Chinese medicine for several centuries. In recent years, the quality and quantity of wild B. striata plants have declined sharply due to habitat deterioration and human over-exploitation. Therefore, it is of great urgency to evaluate and protect B. striata wild plant resource. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to assess the level and pattern of genetic diversity in twelve populations of B. striata. The results showed a high level of genetic diversity (PPB = 90.48%, H = 0.349 4, I = 0.509 6) and moderate genetic differentiation among populations (G(st) = 0.260 9). Based on the unweighted pair-group method with arithmetic average (UPGMA), twelve populations gathered in three clusters. The cluster 1 included four populations. There are Nanjing, Zhenjiang, Xuancheng and Hangzhou. The seven populations which come from Hubei Province, Hunan Province, Jiangxi Province and Guizhou Province belonged to the cluster 2. The cluster 3 only contained Wenshan population. Moreover, Mantel test revealed significant positive correlation between genetic distances and geographic distances (r = 0.632 9; P < 0.000 1). According to the results, we proposed a series of conservation consideration for B. striata.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Plantas Medicinais
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Variação Genética
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Marcadores Genéticos
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China
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Orchidaceae
/
Genética
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Genética Populacional
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2016
Tipo de documento:
Article