Expression and significance of Notch-1 and Jagged-2 in patients with Hirschsprung disease / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 768-771, 2011.
Article
em Zh
| WPRIM
| ID: wpr-321239
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of Notch-1 and Jagged-2 in the normal and spastic segments of colon in patients with Hirschsprung disease(HD), and to explore the correlation of Notch-1 and Jagged-2 with pathogenesis of HD.</p><p><b>METHODS</b>From 2005 to 2010, resected colon specimens of 30 cases with HD were selected for this study. Normal colonic segments were served as control group, while the transitional and spastic segments as experimental group. Immunohistochemical staining, Western blotting, and RT-PCR were applied to detect the expression of Notch-1 and Jagged-2.</p><p><b>RESULTS</b>A large number of Notch-1 and Jagged-2 positive gangliocytes were observed in the control group, while none was observed in spastic segments. Significantly less Notch-1 and Jagged-2 positive gangliocytes were found in the transitional segments. Western blotting revealed that Notch-1 and Jagged-2 protein levels in spastic segments (0.19±0.02 and 0.13±0.04) were less than that in transitional segments and normal segments (0.58±0.05 and 0.52±0.04, 0.72±0.04 and 0.69±0.04, respectively)(P<0.05). RT-PCR revealed that Notch-1 and Jagged-2 mRNA levels were consistent with protein expression.</p><p><b>CONCLUSION</b>Notch-1 and Jagged-2 are not expressed in spastic colon segments, which may be associated with the pathogenesis of HD.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
RNA Mensageiro
/
Transdução de Sinais
/
Estudos de Casos e Controles
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Receptor Notch1
/
Proteína Jagged-2
/
Genética
/
Doença de Hirschsprung
/
Proteínas de Membrana
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Infant
/
Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2011
Tipo de documento:
Article