HPLC-fingerprint-based quality evaluation on a Tibetan medicine Phyllanthus emblica and its tannin parts / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1173-1178, 2014.
Article
em Zh
| WPRIM
| ID: wpr-321343
Biblioteca responsável:
WPRO
ABSTRACT
This study is to establish the fingerprint for Phyllanthus emblica and their tannin parts from different habitats by HPLC for its quality control. The determination was carried out on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column, with methanol-0.2% glacial acetic acid as mobile phase with gradient elution at a flow rate of 1 mL x min(-1). The temperature was maintained at 30 degrees C and the detected wavelength is 260 nm, Thirteen chromatographic peaks were extracted as the common peaks of the fingerprint of P. emblica, and eleven as the common peaks of P. emblica tannin parts, and five peaks were identified by comparing with referent samples. The fingerprints of 8 samples were compared and classified by similarity evaluation, cluster analysis and principal component analysis (PCA). The similarity degrees of eight P. emblica were between 0.763 and 0.993, while tannin parts were between 0.903 and 0.991. All the samples of P. emblica and their tannin parts were classified into 3 categories. The method was so highly reproducible, simple and reliable that it could provide basis for quality control and evaluation of P. emblica from different habitats.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Controle de Qualidade
/
Taninos
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Medicamentos de Ervas Chinesas
/
Química
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Cromatografia Líquida de Alta Pressão
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Classificação
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Tibet
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Phyllanthus emblica
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Medicina Tradicional Tibetana
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article