Establishment of Caco-2 cell monolayer model with collagen coating 6-well plates for study of traditional Chinese medicine prescription / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 515-519, 2014.
Article
em Zh
| WPRIM
| ID: wpr-321394
Biblioteca responsável:
WPRO
ABSTRACT
Caco-2 cell monolayer model is widely utilized in drug absorption study and 12-well transwellTM plates were commonly used to study the absorption of different kinds of natural products. To establish a stable method for the study of traditional Chinese medicine prescription, 6-well plates were chosen because of the larger well volumes than 12-well plates. To study the impacts of collagen kinds, coating density as well as coating time on the cell culture, the transepithelial electrical resistance of Caco-2 cell monolayers grown on different collagen coating transwells was determined, and the permeations of propranolol and atenolol as standard markers were detected with HPLC. The results showed that the kinds of collagen, the different coating densities and coating time of rat tail collagen had no significant influences on the Caco-2 cell monolayer integrality and absorption capacity. 6-well plates coated with 2 micro g Scm-2 rat tail collagen for 1 hour were enough reliable and suitable for the study of traditional Chinese medicine prescription in vitro.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Prescrições de Medicamentos
/
Fatores de Tempo
/
Química
/
Colágeno
/
Técnicas de Cultura de Células
/
Células CACO-2
/
Adsorção
/
Medicina Tradicional Chinesa
/
Métodos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article