Construction and expression of hepatitis B virus envelope protein combined with core protein with two multiple cloning sites vector / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 313-315, 2009.
Article
em Zh
| WPRIM
| ID: wpr-325556
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a coexpression plasmid which expressing envelope protein and nucleoprotein of hepatitis B virus and know of its expressing efficiency.</p><p><b>METHODS</b>The plasmid coexpressing envelope protein and nucleoprotein of hepatitis B virus under the CMV promoter respectively was constructed by gene recombination. Cellular expression was assessed by ELISA.</p><p><b>RESULTS</b>Multiple cloning site was inserted into expression vector contain hepatitis B virus PreS2-S gene. And expression unit containing hepatitis B virus PreC-C was cloned into it. HBsAg and HBeAg was detected both in the culture supernatant and in the cells.</p><p><b>CONCLUSION</b>The coexpressing plasmid was constructed successfully and it can express effectively in vitro. This has provided a basis for further research of the therapeutic HBV DNA vaccine.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Expressão Gênica
/
Vírus da Hepatite B
/
Clonagem Molecular
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Células Hep G2
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Vetores Genéticos
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Genética
/
Antígenos do Núcleo do Vírus da Hepatite B
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Antígenos de Superfície da Hepatite B
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2009
Tipo de documento:
Article