Isolation and analysis of a high expression promoter in rice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 836-840, 2007.
Article
em Zh
| WPRIM
| ID: wpr-327938
Biblioteca responsável:
WPRO
ABSTRACT
The expression of plant gene is controlled by its promoter. The isolation and the function analysis of promoter are important for studying the genetic engineering and the regulation expression of plant genes. In this paper, we cloned a promoter, 0s252, which was predicted to be highly expressed in the stem of rice from the EST database. After the construction of the Os252::GUS expression vector, it was transformed into rice. The integration of transgenes into transgenic rice genome was confirmed through PCR analysis. X-Gluc staining showed that Os252 can promote GUS gene expression in leaf, stem and matured seed. GUS enzyme activities driven by Os252 promoter in leaf and seed are about 190% and 250% of that driven by the 35S promoter. Thus, the Os252 promoter can be applied for rice genetic engineering.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas de Plantas
/
Oryza
/
Regiões Promotoras Genéticas
/
Plantas Geneticamente Modificadas
/
Regulação da Expressão Gênica de Plantas
/
Genética
/
Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Article