microRNA-218 Inhibits Oxygen-induced Retinal Neovascularization via Reducing the Expression of Roundabout 1 / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 709-715, 2016.
Article
em En
| WPRIM
| ID: wpr-328170
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The mechanisms of pathological retinal neovascularization (RNV) remain unknown. Several microRNAs were reported to be involved in the process of RNV. Oxygen-induced retinopathy (OIR) is a useful model to investigate RNV. Our present work explored the expression and the role of microRNA-128 (miR-218) in oxygen-induced RNV.</p><p><b>METHODS</b>OIR was used to establish RNV model. The expression level of miR-218 in the retina from OIR mice was assessed by quantitative real-time reverse transcriptase polymerase chain reaction. Fluorescein angiography was performed in retinae of OIR mice, and RNV was quantified by hematoxylin and eosin staining to evaluate the effect of pCDH-CMV-miR-218 intravitreal injection on RNV in OIR mice. Roundabout 1 (Robo1) expression was detected by Western blotting in mouse retinal vascular endothelial cells expressing a high or low level of miR-218 and retinal tissues from OIR mice. Cell migration was evaluated by scratch wound assay.</p><p><b>RESULTS</b>In OIR mice, the expression level of miR-218 was significantly down-regulated (P = 0.006). Retinal Robo1 expression was significantly increased at both mRNA and protein levels (P = 0.001, 0.008; respectively). miR-218 intravitreal injection inhibited retinal angiogenesis in OIR mice, and the restoration of miR-218 in retina led to down-regulation of Robo1.</p><p><b>CONCLUSIONS</b>Our experiments showed that restoration of miR-218 inhibited retinal angiogenesis via targeting Robo1. MiR-218 contributed to the inhibition of retinal angiogenesis and miR-218 might be a new therapeutic target for preventing RNV.</p>
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1
Base de dados:
WPRIM
Assunto principal:
Oxigênio
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Farmacologia
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Fisiologia
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Receptores Imunológicos
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Neovascularização Retiniana
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Movimento Celular
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Células Cultivadas
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MicroRNAs
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Camundongos Endogâmicos C57BL
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Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2016
Tipo de documento:
Article