Involvement of mitochondria apoptotic pathway in the manumycin inducing apoptosis of U937 and HL-60 / 中华血液学杂志
Chinese Journal of Hematology
; (12): 404-406, 2007.
Article
em Zh
| WPRIM
| ID: wpr-328331
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis induced by manumycin in U937 and HL-60 cell lines, and to explore the role of mitochondria apoptotic pathway in manumycin-inducing apoptosis.</p><p><b>METHODS</b>Leukemic cells line U937 and HL-60 were treated by manumycin at 2 micromol/L for different time. Apoptosis of leukemia cells was detected by flow cytometry. The cytosolic proteins were extracted using a digitonin buffer. The protein expression of cytochrome C, caspase-9, caspase-8, and caspase-3 were determined by western blot. Mitochondrial membrane potential was detected by JC-1.</p><p><b>RESULTS</b>In U937 and HL-60 cells, manumycin induced mitochondrial depolarization after 6 h treatment. The average red/green fluorescence ratios at 6 h were significantly (P < 0.01) lower than those at time 0, being 0.51 +/- 0.07 and 0.41 +/- 0.06 for control group respectively. Manumycin induced cytochrome C release from the mitochondria into the cytosol after 6 h treatment, and activated caspase-9, caspase-8, and caspase-3 after a 16h treatment. The broad-spectrum caspase-inhibitor Z-VAD-fmk at 50 micromol/L was able to inhibit caspase cleavage completely, but only reduced the manumycin-induced apoptosis rates by 51.69% and 56.47% in U937 and HL-60, respectively.</p><p><b>CONCLUSION</b>Manumycin induced apoptosis in U937 and HL-60 cell lines via mitochondria apoptotic pathway.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
/
Polienos
/
Apoptose
/
Células HL-60
/
Citocromos c
/
Alcamidas Poli-Insaturadas
/
Caspase 3
/
Caspase 8
/
Caspase 9
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2007
Tipo de documento:
Article