Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer extension preamplification / 中华血液学杂志
Chinese Journal of Hematology
; (12): 687-689, 2006.
Article
em Zh
| WPRIM
| ID: wpr-328398
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for identifying fetal nucleated erythrocytes (NRBCs) in maternal blood.</p><p><b>METHODS</b>NRBCs in maternal blood were detected by benzidine staining and collected by micromanipulation. After primer extension preamplification (PEP) of the entire genome from a single NRBC, short tandem repeat (STR) genotype was analysed after further amplification of this gene. Single NRBC was differentiated as fetal or maternal origin by comparison of STR genotype of NRBC with its corresponding parents.</p><p><b>RESULTS</b>NRBCs were found in all of 28 pregnant women in a range of 4 to 13 per 5 ml venous blood. About 43. 6% of NRBCs were determined to be fetal origin by STR typing.</p><p><b>CONCLUSION</b>This method provides effective identification of fetal NRBCs and allows non-invasive prenatal genetic diagnosis using single fetal NRBC.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Diagnóstico Pré-Natal
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Eritroblastos
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Talassemia beta
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Repetições de Microssatélites
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Análise de Sequência com Séries de Oligonucleotídeos
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Biologia Celular
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Diagnóstico
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Contagem de Eritrócitos
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Feto
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Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
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Female
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Humans
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Male
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Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2006
Tipo de documento:
Article