The Rapid Establishment of Human Clonal Adipose Derived Stem Cell (hADSC) Lines with Aspirated Adipose Tissue
Journal of Korean Burn Society
; : 93-96, 2011.
Article
em Ko
| WPRIM
| ID: wpr-32897
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: This study aims to establish a new strategy that provides for the rapid establishment of human clonal adipose derived stem cell (hADSC) lines with aspirated adipose tissue and to characterize newly generated hMSC lines for their cell phenotype, differentiation potential, lineage-specific gene expression. METHODS: Human adipose tissue-derived stem cells (hADSCs) were isolated from subcutaneous adipose tissue based on standard protocols. After incubation for 2 h, only the cell culture supernatant was transferred to a new dish. This process was repeated several times with 30 h incubations. RESULTS: We confirmed the difference in growth rate, however, differences were not seen in the differentiation capabilities and stemness of the each cell lines. CONCLUSION: It is necessary to establish cell lines via single cell level for application to disease specific tissue engineering.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fenótipo
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Células-Tronco
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Linhagem Celular
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Tecido Adiposo
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Técnicas de Cultura de Células
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Engenharia Tecidual
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Gordura Subcutânea
Limite:
Humans
Idioma:
Ko
Revista:
Journal of Korean Burn Society
Ano de publicação:
2011
Tipo de documento:
Article