Optimization of the method for isolating and culturing rat mesenchymal stem cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1621-1631, 2014.
Article
em Zh
| WPRIM
| ID: wpr-329235
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To optimize the protocols for isolation and culture of mesenchymal stem cells from rat bone marrow (BMSCs).</p><p><b>METHODS</b>BMSCs were isolated by adherence to plastic with frequent medium change and reduced trypsinization time. The cell growth curves were drawn and the surface markers of BMSCs were detected by flow cytometry. The cells were induced to differentiate into osteogenic, adipogenic, hepatic and cholic lineages.</p><p><b>RESULTS</b>The cells isolated using this method were positive for CD29, CD44, and CD90 and negative for the hematopoietic surface markers CD45. The osteogenic and adipogenic differentiation of the BMSCs was verified by alkaline phosphatase staining, Alizarin red staining and Oil red staining. The cell subcultures up to passage 10 maintained capacities of differentiation into osteogenic and adipogenic lineages. The BMSCs induced with sequential addition of growth factors, cytokines and hormones differentiated into cells expressing hepatocyte- and cholangiocyte-specific markers.</p><p><b>CONCLUSION</b>The optimized method allows efficient isolation of homogenous populations of MSCs from rat bone marrow, which can be induced into multiple cell lineages.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Diferenciação Celular
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Separação Celular
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Técnicas de Cultura de Células
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Biologia Celular
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Proliferação de Células
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Células-Tronco Mesenquimais
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Citometria de Fluxo
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Article