LPS-induced degeneration of dopaminergic neurons of substantia nigra in rats / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 83-86, 2004.
Article
em En
| WPRIM
| ID: wpr-330865
Biblioteca responsável:
WPRO
ABSTRACT
In order to investigate the neurotoxicity of lipopolysaccharide (LPS) on the dopaminergic neurons of substantia nigra and the pathogenesis of Parkinson disease, LPS was stereotaxically infused into substantia nigra (SN). At different dosages and different time points with 5 microg LPS, the damage of the dopaminergic neurons in SN was observed by using tyrosine-hydroxylase (TH) immunohistochemical staining. The results showed that 14 days after injection of 0.1 microg to 10 microg LPS into the rat SN, TH-positive (TH+) neurons in the SN were decreased by 5%, 15%, 20%, 45 %, 96% and 99% respectively. After injection of 5 microg LPS, as compared with the control groups, TH+ neurons began to decrease at 3rd day and obviously decrease at 14th day, only 5% of total cells, and almost disappeared 30 days later. The results suggested that LPS could induce the degeneration of dopaminergic neurons in the SN in a dose- and time-dependent manner.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Doença de Parkinson Secundária
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Patologia
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Substância Negra
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Dopamina
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Distribuição Aleatória
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Lipopolissacarídeos
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Ratos Sprague-Dawley
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Relação Dose-Resposta a Droga
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Toxicidade
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Metabolismo
Limite:
Animals
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2004
Tipo de documento:
Article