Effect of lentinan against immunosuppression of lymphocytes cultured in simulated microgravity environment / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 182-186, 2012.
Article
em Zh
| WPRIM
| ID: wpr-330994
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to evaluate the effect of lentinan on the immune function of splenic lymphocytes in rotary cell culture system (RCCS) microgravity environment. The splenic lymphocytes from mice were separated and cultured in the normal gravity and the microgravity environments. The cells were treated with lentinan solution (0, 10, 20 and 40 µg/ml). After incubated with lentinan for indicated times (24, 48 and 72 h), the cell proliferation, secretion of cytokine and the expression of cell surface markers were detected by MTT method, ELISA and flow cytometry respectively. The results indicated that lentinan of above mentioned concentrations did not obviously promote the lymphocyte proliferation, but increased the secretion of IL-2 and IFN-γ and enhanced the expression of lymphocyte surface markers CD4 and CD8 in microgravity environment. It is concluded that lentinan has the ability to enhance the lymphocyte immune function in microgravity environment.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Baço
/
Ativação Linfocitária
/
Linfócitos
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Células Cultivadas
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Citocinas
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Terapia de Imunossupressão
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Simulação de Ausência de Peso
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Secreções Corporais
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Biologia Celular
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2012
Tipo de documento:
Article