The preliminary analysis on immunogenicity of DNA vaccines against human papillomavirus 58 / 病毒学报
Chinese Journal of Virology
; (6): 196-201, 2008.
Article
em Zh
| WPRIM
| ID: wpr-334824
Biblioteca responsável:
WPRO
ABSTRACT
In order to evaluate the immunogenicity of HPV 58 L1 DNA vaccines, five DNA vaccines had been constructed with pcDNA3.1 vector containing different L1 genes of HPV 58, which were designated as L1h, L1hDeltac, L1S, L1SM and L1wt. The protein expression of DNA vaccines in vitro was tested by Western blot. The ability of forming pseudovirus was evaluated by transfecting DNA vaccine together with pcDNA3.1-h58L2 and pcDNA3.1-GFP into 293FT cells. The neutralizing antibodies and cellular immune response produced in BALB/c mice immunized with the DNA vaccines were detected by using pseudovirus-based neutralization assay and ELISPOT respectively. The results showed that the five DNA vaccines had been successfully constructed; the level of protein expression of L1hDeltac was the highest and those for L1S and L1SM were of medium, while no expressed target protein of L1wt was detected. Only L1S could form the pseudovirus while the other four vaccines could not. L1S and L1h could induce neutralizing antibody. However, the average titer of neutralizing antibody for L1S (16,400) was much higher than that for L1h (148) and the other three vaccines could not induce neutralizing antibody. No cellular immune response for all five DNA vaccines was detectable by ELISPOT. The results indicated that DNA vaccine against HPV 58 can form pseudovirus in vitro, also can induce high level of neutralizing antibodies. This provides reference for screening HPV vaccine in future.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Papillomaviridae
/
Proteínas Recombinantes de Fusão
/
Ensaio de Imunoadsorção Enzimática
/
Testes de Neutralização
/
Transfecção
/
Linhagem Celular
/
Western Blotting
/
Imunização
/
Vacinas de DNA
/
Proteínas de Fluorescência Verde
Limite:
Animals
/
Female
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2008
Tipo de documento:
Article
País de publicação:
CHINA
/
CN
/
REPUBLIC OF CHINA