Silencing HSV1 gD expression in cultured cells by RNA interference / 病毒学报
Chinese Journal of Virology
; (6): 22-27, 2007.
Article
em Zh
| WPRIM
| ID: wpr-334915
Biblioteca responsável:
WPRO
ABSTRACT
To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas Recombinantes de Fusão
/
Células Vero
/
Transfecção
/
Chlorocebus aethiops
/
Proteínas do Envelope Viral
/
Reação em Cadeia da Polimerase
/
Herpesvirus Humano 1
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Proteínas de Fluorescência Verde
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2007
Tipo de documento:
Article