Effects of temperature and pH on the growth of H1N1 subtype of influenza A virus by surface-enhanced Raman spectroscopy / 生物工程学报
Chinese Journal of Biotechnology
; (12): 447-456, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-337452
Biblioteca responsável:
WPRO
ABSTRACT
Surface enhanced Raman spectroscopy technology (SERS), using gold nanoparticles as a base, was developed for rapid and sensitive detection of virus strains. SERS can be used as a rapid and reliable method to distinguish the titers of viral replication. In the present study, we characterized H1N1 subtypes of influenza A virus strains in different conditions of pH or temperatures, while we analyzed data from SERS technology using gold nanoparticles as a base and cell cultures were employed to further confirm the data from virus strains. Origin8.0 was used to collect Raman spectra, smooth and homogenize data, and to contrast spectra. Our results indicated that the peaks of different virus strains in optimal environmental conditions (T=37 ℃/pH=7.2) reached ≥3 000. This criterion was verified by subsequent virological method. The present data indicate that the established SERS protocol can be used as a rapid and reliable method to distinguish the replication rate of virus, which can be further used in clinical samples.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Análise Espectral Raman
/
Temperatura
/
Cultura de Vírus
/
Vírus da Influenza A Subtipo H1N1
/
Nanopartículas
/
Ouro
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Concentração de Íons de Hidrogênio
/
Métodos
Tipo de estudo:
Guia de prática clínica
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2016
Tipo de documento:
Artigo