Nacre-induced osteogenesis in the femoral condyles of New Zealand rabbits / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate nacre powder-induced osteogensis in the femoral condyles of New Zealand rabbits and investigate the possible mechanism.</p><p><b>METHODS</b>Nacre powder was implanted into the femoral condyles of New Zealand rabbits and 4, 8, and 12 weeks after the implantation, radiographic examinations were carried out and the bone density was evaluated. The bone tissue specimens were sliced after fixation for histological observations. The osteogenesis area on the slice was estimated with Ponceau Red staining 8 and 12 weeks after the implantation and calculated with Imaga-Pro software.</p><p><b>RESULTS</b>X-ray after the operation did not reveal obvious evidence of angiogenesis in the femoral condyles, where the X-ray density underwent slight changes. The optical density decreased significantly after the implantation, and the quantity of the osteoid, woven and lamellar bone increased in the bone tissue with time. The osteogenesis area with Ponceau Red staining showed obvious bone formation, which was significantly different from the control group.</p><p><b>CONCLUSION</b>T Obvious osteogenesis occurred in the femoral condyles after nacre powder implantation in New Zealand rabbits. Nacre powder has slow biodegradation in vivo and induces osteogenesis by osteoinduction.</p>