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High-performance capillary electrophoresis for determining the chemical compositions of Gegen and Fenge extracts / 南方医科大学学报
Article em Zh | WPRIM | ID: wpr-340809
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for analyzing the chemical compositions of Gegen and Fenge extracts using high-performance capillary electrophoresis (HPCE).</p><p><b>METHODS</b>Using HPCE/DAD, the chemical composition of the extracts was analyzed with the buffer solution of 40 mmol/L borax containing 16.7% methanol, with injection pressure at 137.9 kPa for 5 s, separation voltage at 25 kV in 0-5 min time range and at 22 kV in 5-25 min time range, and the temperature of the capillary of 20 degrees celsius.</p><p><b>RESULTS AND CONCLUSION</b>The method for analysis of Gegen and Fenge extracts was established, which identified puerarin and daidzein as the two major components. This simple and rapid analysis method can be used for Gegen and Fenge extract fingerprinting.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Química / Reprodutibilidade dos Testes / Eletroforese Capilar / Pueraria / Isoflavonas / Métodos Tipo de estudo: Prognostic_studies Idioma: Zh Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Química / Reprodutibilidade dos Testes / Eletroforese Capilar / Pueraria / Isoflavonas / Métodos Tipo de estudo: Prognostic_studies Idioma: Zh Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Article