TNF-alpha induced reversal of multidrug resistance in human hepatocellular carcinoma cells / 中华外科杂志
Chinese Journal of Surgery
; (12): 602-604, 2007.
Article
em Zh
| WPRIM
| ID: wpr-342113
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the reversal of multidrug resistance in the cell line HepG2/ADM induced by TNF-alpha.</p><p><b>METHODS</b>HepG2/ADM cells were incubated with different concentrations of TNF-alpha (100, 500 and 2500 U/ml) for 72 h. Real-time PCR was performed to compare the mRNA levels of MDR1 with PPAR-alpha in the different concentrations of TNF-alpha treated cells. The Annexin V assay was used to check cell apoptosis induced by 0.5 mg/L adriamycin. Rhodamine 123 efflux assay and MTT assay were used to study P-gp activity and drug resistance in each group, respectively.</p><p><b>RESULTS</b>TNF-alpha could induce down-regulation of MDR1 and up-regulation of PPAR-alpha. Meanwhile, it could enhance cell cytotoxicity and cell apoptosis induced by 0.5 mg/L adriamycin.</p><p><b>CONCLUSIONS</b>TNF-alpha could partially reverse the multidrug resistance of HepG2/ADM cells by down-regulating the expression of MDR1 and up-regulating the expression of PPAR-alpha.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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RNA Mensageiro
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Doxorrubicina
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Fator de Necrose Tumoral alfa
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Apoptose
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Carcinoma Hepatocelular
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Membro 1 da Subfamília B de Cassetes de Ligação de ATP
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Resistência a Múltiplos Medicamentos
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Resistencia a Medicamentos Antineoplásicos
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Ano de publicação:
2007
Tipo de documento:
Article