Silencing of ABCG2 by MicroRNA-3163 Inhibits Multidrug Resistance in Retinoblastoma Cancer Stem Cells
Journal of Korean Medical Science
; : 836-842, 2016.
Article
em En
| WPRIM
| ID: wpr-34240
Biblioteca responsável:
WPRO
ABSTRACT
To investigate the function and regulation mechanism of ATP-binding cassette, subfamily G, member 2 (ABCG2) in retinoblastoma cancer stem cells (RCSCs), a long-term culture of RCSCs from WERI-Rb1 cell line was successfully established based on the high expression level of ABCG2 on the surface of RCSCs. To further explore the molecular mechanism of ABCG2 on RCSCs, a microRNA that specifically targets ABCG2 was predicted. Subsequently, miR-3163 was selected and confirmed as the ABCG2-regulating microRNA. Overexpression of miR-3163 led to a significant decrease in ABCG2 expression. Additionally, ABCG2 loss-of-function induced anti-proliferation and apoptosis-promoting functions in RCSCs, and multidrug resistance to cisplatin, carboplatin, vincristine, doxorubicin, and etoposide was greatly improved in these cells. Our data suggest that miR-3163 has a significant impact on ABCG2 expression and can influence proliferation, apoptosis, and drug resistance in RCSCs. This work may provide new therapeutic targets for retinoblastoma.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Retinoblastoma
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Células-Tronco Neoplásicas
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Sequência de Bases
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Transfecção
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Alinhamento de Sequência
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Apoptose
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Resistencia a Medicamentos Antineoplásicos
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Regiões 3' não Traduzidas
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Inativação Gênica
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MicroRNAs
Limite:
Humans
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2016
Tipo de documento:
Article