Effect of p21-targeted shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 1102-1106, 2009.
Article
em Zh
| WPRIM
| ID: wpr-344050
Biblioteca responsável:
WPRO
ABSTRACT
In the present study, shRNA plasmid of pSi-p21 targeting p21 mRNA was constructed and the effect of p21 shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells was investigated. The effect of curcumin on the expression of p21 mRNA and protein and the silence efficiency of pSi-p21 were detected with RT-PCR and Western blotting. The effect of pSi-p21 on curcumin-induced apoptosis of Huh7 cells was evaluated with DAPI staining. The results showed that curcumin significantly upregulated p21 mRNA and protein expression, which was knocked down by pSi-p21 of Huh7 cells. DAPI staining results showed that pSi-p21 significantly decreased curcumin-induced apoptosis of Huh7 cells. The data suggested that curcumin induced apoptosis of Huh7 cells via upregulation of p21 expression.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Plasmídeos
/
RNA Mensageiro
/
Transfecção
/
Regulação Neoplásica da Expressão Gênica
/
Regulação para Cima
/
Apoptose
/
Carcinoma Hepatocelular
/
Curcumina
Limite:
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2009
Tipo de documento:
Article