Flow Cytometric White Blood Cell Differential Using CytoDiff is Excellent for Counting Blasts
Annals of Laboratory Medicine
; : 28-34, 2015.
Article
em En
| WPRIM
| ID: wpr-34579
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: The usefulness of the CytoDiff flow cytometric system (Beckman Coulter, USA) has been studied in various conditions, but its performance including rapidity in detecting and counting blasts, the most significant abnormal cells in the peripheral blood, has not been well evaluated. The objective of this study was to evaluate the performance of the CytoDiff differential counting method in challenging samples with blasts. METHODS: In total, 815 blood samples were analyzed. Samples flagged as "blasts" or "variant lymphocytes" and showing 0.8) for neutrophils and lymphocytes but poor (r<0.8) for other cells. When the cutoff value of the CytoDiff blast count was set at 1%, the sensitivity was 94.4% (95% CI; 91.2-96.6) and specificity was 91.9% (95% CI; 89.0-94.1). The positive predictive value was 88.4% (95% CI; 84.4-91.5) (304/344 cases) and negative predictive value was 96.2% (95% CI; 93.9-97.7) (453/471 cases). The CytoDiff blast counts correlated well to the manual counts (r=0.9223). CONCLUSIONS: The CytoDiff method is a specific, sensitive, and rapid method for counting blasts. A cutoff value of 1% of at least 1 type of blast is recommended for positive CytoDiff blast counts.
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Base de dados:
WPRIM
Assunto principal:
Linfócitos
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Citometria de Fluxo
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Contagem de Leucócitos
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Leucócitos
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Neutrófilos
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2015
Tipo de documento:
Article