Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappa B pathway in human dermal fibroblasts
Experimental & Molecular Medicine
; : 548-554, 2009.
Article
em En
| WPRIM
| ID: wpr-34745
Biblioteca responsável:
WPRO
ABSTRACT
Cordycepin (3'-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappa B activity, which was determined by I kappa B alpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappa B binding activity. However, UVB-induced NF-kappa B activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappa B activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.
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Base de dados:
WPRIM
Assunto principal:
Pele
/
Raios Ultravioleta
/
Envelhecimento
/
Regulação Enzimológica da Expressão Gênica
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Células Cultivadas
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Indução Enzimática
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Desoxiadenosinas
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NF-kappa B
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Metaloproteinase 3 da Matriz
/
Derme
Limite:
Humans
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Male
/
Newborn
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2009
Tipo de documento:
Article