Different subtypes of estrogen receptor α and related signal molecules in the hippocampus are associated with spatial cognitive impairment of diabetic mice / 生理学报
Acta Physiologica Sinica
; (6): 252-260, 2017.
Article
em Zh
| WPRIM
| ID: wpr-348277
Biblioteca responsável:
WPRO
ABSTRACT
To study the correlation between the spatial cognitive impairment and different subtypes of estrogen receptor α (ERα) of hippocampus in diabetic mice, we used alloxan (intraperitoneal injection) to induce type 1 diabetes in male Kunming mice and compared the spatial cognitive ability of the model mice with that of control mice through Morris water maze test. Meanwhile, using Western blot, we detected the protein expressions of ER-α36, ER-α66, caveolin-1, PKCα, cAMP-response element binding protein 2 (CREB2), and synaptophysin (Syn) in the hippocampus of the mice. The results showed that on the 3rd and 5th days of training, the ability of spatial learning and memory in the diabetic mice was significantly inferior to that of the control mice (P < 0.05). In the diabetic mice, the protein expressions of caveolin-1 and PKCα were decreased (P < 0.05), but ER-α66 expression was unaffected, while ER-α36 and CREB2 expressions were significantly increased (P < 0.05) compared with those of the control mice. The results suggest that abnormal expression of ER-α36 and related signal molecules may be important factors for diabetes-induced spatial cognitive impairment.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Sinaptofisina
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Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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Aprendizagem em Labirinto
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Receptor alfa de Estrogênio
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Diabetes Mellitus Experimental
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Proteína Quinase C-alfa
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Caveolina 1
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Disfunção Cognitiva
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Hipocampo
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Memória
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Ano de publicação:
2017
Tipo de documento:
Article