Effect of carbon disulfide exposure on fatty acid metabolism in ApoE knockout and C57BL/6J mice / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 538-540, 2015.
Article
em Zh
| WPRIM
| ID: wpr-350549
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the influences of carbon disulfide (CS2) exposure on fatty acid metabolism in apolipoprotein E (ApoE) knockout mice and C57BL/6J mice.</p><p><b>METHODS</b>Twenty-four male ApoE knockout mice were randomly and equally divided into four groups: a CS2-exposed normal diet group, a CS2-unexposed normal diet group, a CS2-exposed high-fat diet group, and a CS2-unexposed high-fat diet group. Twenty-four C57BL/6J male mice were divided into four groups in the same way. The CS2-exposed groups were exposed to CS2 (1 g/m(3)) by static inhalation for 5 hours a day, 5 days a week. After two weeks, the whole blood of mice was collected. Methyl ester derivatization of fatty acids was performed using an acid-catalyzed method. Fatty acid contents before and after exposure were compared by gas chromatography-mass spectroscopy.</p><p><b>RESULTS</b>There were significant differences in fatty acid contents of mice between the four groups. For the C57BL/6J mice, the arachidic acid contents in the CS2-exposed high-fat diet group were significantly lower than those in the CS2-unexposed high-fat diet group (P = 0.045 0). For the ApoE knockout mice, the arachidonic acid contents in the CS2-exposed normal diet group were significantly lower than those in the CS2-unexposed control diet group (P = 0.045 2). For the ApoE knockout mice, the γ-linolenic acid contents in the CS2-exposed high-fat diet group were significantly higher than those in the unexposed high-fat diet group (P = 0.044 7).</p><p><b>CONCLUSION</b>Exposure to CS2 can induce fatty acid metabolism disorder in mice, indicating that CS2 may increase the risk of atherosclerosis and other cardiovascular diseases.</p>
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Base de dados:
WPRIM
Assunto principal:
Apolipoproteínas E
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Administração por Inalação
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Dissulfeto de Carbono
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Química
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Camundongos Knockout
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Aterosclerose
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Metabolismo dos Lipídeos
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Toxicidade
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Ácidos Graxos
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Dieta Hiperlipídica
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2015
Tipo de documento:
Article