Expression and crystallographic studies of a fungal immunomodulatory protein LZ-8 from a medicinal fungus Ganoderma lucidum / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1563-1568, 2010.
Article
em Zh
| WPRIM
| ID: wpr-351561
Biblioteca responsável:
WPRO
ABSTRACT
LZ-8 protein, isolated from a well known Chinese traditional medicinal fungus Ganoderma lucidum, is the first member of fungal immunomodulatory protein, members of which have been isolated from a variety of medicinal and edible mushrooms in the last two decades. The protein plays a multifunctional and important role in modulating immune system. In this report, in order to get LZ-8 protein crystals, the LZ-8 gene was expressed and purified by affinity chromatography, gel filtration chromatography and anion exchange chromatography subsequently. The protein was then crystallized using the hanging-drop vapour-diffusion method. The LZ-8 crystals were obtained and the phase information was calculated by X-ray diffraction. The resolution of LZ-8 crystals is 3.2A. This study will provide an insight into the structure of fungal immunomodulatory proteins.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas Recombinantes
/
Proteínas Fúngicas
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Cristalografia
/
Ganoderma
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Alergia e Imunologia
/
Vetores Genéticos
/
Genética
/
Fatores Imunológicos
/
Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Article