Degradation of 14-3-3beta appeared in apoptosis cell induced by PrP106-126 polypeptide / 病毒学报
Chinese Journal of Virology
; (6): 414-417, 2012.
Article
em Zh
| WPRIM
| ID: wpr-354715
Biblioteca responsável:
WPRO
ABSTRACT
To investigate changes of 14-3-3beta from apoptosis induced by PrP106-126 polypeptide, HeLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3beta was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3beta appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3beta mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 143-3beta induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fragmentos de Peptídeos
/
Farmacologia
/
Príons
/
Células HeLa
/
Apoptose
/
Proteínas 14-3-3
/
Proteólise
/
Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Article