Genetic characteristics of EV71 in Yunnan Province from 2009 to 2010 / 病毒学报
Chinese Journal of Virology
; (6): 389-393, 2012.
Article
em Zh
| WPRIM
| ID: wpr-354719
Biblioteca responsável:
WPRO
ABSTRACT
In order to learn about the genetic characteristic of human enterovirus type71 (HEV71) isolated from cases of Hand, Foot and Mouth Disease (HFMD) in Yunnan Province from 2009 to 2010. 50 isolates form HFMD cases were performed entire VP1 coding region amplification by reverse transcription-polymerase chain reaction and sequencing the nucleotide sequences; then the phylogenetic tree was constructed. The complete nucleotide sequences of region VP1 of the 50 strains were all 891nt length coding 297 amino acids. The result of molecular identification of the 50 strains is HEV71. Phylogenetic analysis indicated that 48 EV71 isolates belonged to subgenotype C4a and 2 EV71 isolates belonged to genotype A. From 2009 to 2010, the pathogen of HFMD cases were EV71 strains in Yunnan province, which were co-evolved with isolates from other provinces in mainland of China. There was no significant difference found in the whole sequence of VP1 gene of the strains isolated from different regions or under different diseases occurred, but the spread of genetype A appared in Yunnan Province in 2009.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Virologia
/
Virulência
/
China
/
Classificação
/
Análise de Sequência de DNA
/
Enterovirus Humano A
/
Proteínas do Capsídeo
/
Técnicas de Genotipagem
/
Genética
Tipo de estudo:
Prognostic_studies
Limite:
Child, preschool
/
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Article