Modulation effects of human immature and mature dendritic cells on glatiramer acetate specific T cell lines in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 409-415, 2003.
Article
em En
| WPRIM
| ID: wpr-355635
Biblioteca responsável:
WPRO
ABSTRACT
A large body of evidence demonstrates that dendritic cells (DC) play a pivotal role in the control of immunity by priming and tolerizing T cells. In multiple sclerosis (MS), autoreactive T cells are proposed to play a pathogenic role by secreting pro-inflammatory cytokines, but comparison studies on the effects of immature and mature dendritic cells on the cytokines profile of antigen-specific T cell lines are lacking. To evaluate the actions of dendritic cell maturation on T cell polarization, the effects of immature and mature dendritic cells derived from MS patients on in vitro proliferative responses, and cytokine production by glatiramer acetate (GA)- specific T cell lines (TCL) derived from MS patients were analyzed. The results demonstrated that it is easy to derive GA-specific TCL from MS patients with high specificity; lipopolysaccharide can efficiently induce DC maturation within 24 hours at a concentration of 5 micro g/ml; mature DC showed higher co-stimulatory capacity of GA-specific TCLs than immature DC. GA-specific TCLs produce dominantly IL-2, IL-4, IFN-gamma and IL-10, but low levels of IL-6. In contrast to immature DC, mature DC enhanced capacity to induce IL-6 and IL-10 secretion, but down-regulate IL-2, IL-4 and IFN-gamma production by GA- specific TCLs. It is concluded that DC maturation status modulating proliferation of TCL and production of cytokines may represent another focus for the study on both immuno-pathogenesis and immunotherapeutic interventions in MS.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Peptídeos
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Fisiologia
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Células Dendríticas
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Ativação Linfocitária
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Linfócitos T
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Linhagem Celular
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Citocinas
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Alergia e Imunologia
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Acetato de Glatiramer
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Esclerose Múltipla
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Article