Dynamic changes of Smac/DIABLO protein expression in rat donor myocardium suffered from hypothermic preservation / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 296-300, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-356271
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the effect of different duration of hypothermic preservation on the expression of Smac/DIABLO protein in rat hearts.</p><p><b>METHODS</b>The Langendorff model of isolated rat heart was used. After stored in 4 degrees C Celsior solution for different time (0, 3, 6, 9 or 12 h), the activity of SOD and the content of MDA in heart mitochondria were measured. Cell apoptosis was detected by TUNEL technique. The expression of Smac/DIABLO protein was also analyzed by Western blotting.</p><p><b>RESULTS</b>(1) After hypothermic preservation, the activity of SOD was decreased and the content of MDA was increased in rat hearts in a time-dependent manner. (2) Prolonged the hypothermic preservation, the percentage of apoptotic cell also enhanced. (3) After long-term of cold preservation, the expression of Smac/DIABLO protein increased at 3-6 h of preservation but decreased after 9 h.</p><p><b>CONCLUSION</b>Prolonged the hypothermic preservation might lead to the expression of Smac/DIABLO protein and induce cardiomyocytes apoptosis, which may in turn result in malfunction of cardiomyocytes.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Preservação de Órgãos
/
Proteínas de Transporte
/
Distribuição Aleatória
/
Transplante de Coração
/
Ratos Sprague-Dawley
/
Temperatura Baixa
/
Proteínas Mitocondriais
/
Coração
/
Metabolismo
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo