Proliferation and apoptosis in vitro of umbilical cord blood CD34+CD38- hematopoietic early progenitor cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 229-234, 2005.
Article
em Zh
| WPRIM
| ID: wpr-356587
Biblioteca responsável:
WPRO
ABSTRACT
To cultivate CD34(+)CD38(-) cells isolated from umbilical cord blood of healthy puerperal women over a longer-period of time for observation of cell division, proliferation, apoptosis, and effects of stem cell factor on the growth of CD34(+)CD38(-) cells, with flow cytometry, CD34(+)CD38(-) cells were isolated from umbilical cord blood of 10 healthy puerperal women and cultivated in stem cell media with supplement of IL-3, IL-6, GM-CSF, EPO, IGF-1 and SCF 6 kinds cell growth stimulating factors for six months. The cell growth curves were established. The effects of stem cell factor on the growth of CD34(+)CD38(-) cells and cell apoptosis were investigated with the single cell gel electrophoresis technique and flow cytometry method, respectively. The results showed that CD34(+)CD38(-) cells isolated from umbilical cord blood were capable of proliferating after being cultivated in vitro over a longer-period of time with no evidence of the presence of excessive apoptosis. In conclusion, under appropriate culture conditions, CD34(+)CD38(-) hematopoietic early progenitor cells from umbilical cord blood can serve as a resource providing a large amount of primitive cells for transplantation therapy after a longer period of cultivation and proliferation in vitro.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Células-Tronco Hematopoéticas
/
Células Cultivadas
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Apoptose
/
Antígenos CD34
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Biologia Celular
/
Proliferação de Células
/
Alergia e Imunologia
/
ADP-Ribosil Ciclase 1
/
Sangue Fetal
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Citometria de Fluxo
Limite:
Adult
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Female
/
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Article