Preparation and Assessment of IL1RAP Monoclonal Antibody / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 962-965, 2015.
Article
em Zh
| WPRIM
| ID: wpr-357238
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare and identify human monoclonal antibody against IL-1 receptor accessory protein (IL1RAP), which is a new identified surface marker for leukemia stem cells (LSC).</p><p><b>METHODS</b>BALB/c mice were inoculated intraperitoneally with hybridoma cells (3H6E10, 10D8A7) and their ascites were collected. The monoclonal antibody against hu-IL1RAP specifically was purified from ascites, the nondenaturing-PAGE, ELISA and Western blot were used to detect the purity, titer and sensitivity of antibody.</p><p><b>RESULTS</b>Two purified antibodies were obtained and named as 3H6E10 McAb and 10D8A7 McAb, whose purity was 95% and 94% respectively. The titer of two purified monoclonal antibodies was 1 81000 and specific conjugation of IL1RAP purified protein and endogenous protein from normal people and leukemia patients with purified antibodies were confirmed.</p><p><b>CONCLUSION</b>The purified monoclonal antibodies which can specifically bind to hu-IL1RAP are successfully prepared, thus providing novel way to effectively clear LSC in the future.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Células-Tronco Neoplásicas
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Ensaio de Imunoadsorção Enzimática
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Leucemia
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Western Blotting
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Proteína Acessória do Receptor de Interleucina-1
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Hibridomas
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Camundongos Endogâmicos BALB C
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Anticorpos Monoclonais
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Especificidade de Anticorpos
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2015
Tipo de documento:
Article