Impact of PI3K /Akt /mdm2 signaling pathway on the sensitivity of gastric cancer cell line SGC7901 to doxorubicin / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 494-497, 2008.
Article
em Zh
| WPRIM
| ID: wpr-357390
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether PI3K/Akt/mdm2 signalling pathway affect the sensitivity of gastric cancer cell line SGC7901 cells to doxorubicin.</p><p><b>METHODS</b>Gastric cancer cell line SGC7901 cells were exposed to doxorubicin and specific PI3K inhibitor wortmannin. Cell apoptosis was detected using flow cytometry. PI3K activity was detected by radioactive immunoprecipitation-kinase assay. Western blotting was employed to evaluate the expressions of PI3K-p85, pAkt-S473, Akt, pmdm2-S166 and p53.</p><p><b>RESULTS</b>The level of apoptosis in gastric cancer SGC7901 cells treated with doxorubicin was gradually increasing. wortmannin enhanced its effects significantly. PI3K activity and the expression of pAkt-S473 increased in a time-dependent manner, pmdm2-S166, p53 were also increased wortmannin inhibited phosphorylation of mdm2 and improved the p53 expression.</p><p><b>CONCLUSION</b>PI3K/Akt/mdm2 signalling pathway can be activated by doxorubicin and suppress apoptosis by promoting phosphorylation of mdm2. PI3K inhibitor wortmannin can enhance sensitivity of gastric cancer cells to chemotherapy.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
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Fosforilação
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Neoplasias Gástricas
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Transdução de Sinais
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Doxorrubicina
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Proteína Supressora de Tumor p53
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Apoptose
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Resistencia a Medicamentos Antineoplásicos
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Fosfatidilinositol 3-Quinases
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2008
Tipo de documento:
Article