Protection against acute hypoxic/reoxygenation injury to kidney for rabbit with morphine hypoxic preconditioning by observing the expression of caspase-3 protein / 生物医学工程学杂志
J. biomed. eng
; Sheng wu yi xue gong cheng xue za zhi;(6): 531-533, 2011.
Article
em Zh
| WPRIM
| ID: wpr-359231
Biblioteca responsável:
WPRO
ABSTRACT
The maintenance of the balance between oxygen supply and oxygen consumption is a key measure in preventing acute kidney hypoxic/reoxygenation injury. Morphine can inhibit metabolism and reduce the oxygen consumption. We tried to investigate the protective effects of morphine hypoxic preconditioning on acute kidney hypoxic/reoxygenation injury in rabbit and its influence on expression of caspase-3 protein. Kidney hypoxic and reoxygenation were induced by making the tested rabbits inhale 8% oxygen for three hours firstly, and then putting them in the air to breathe in normal oxygen for another three hours. Morphine hypoxic preconditioning was induced by administering morphine 3 mg/kg, and then hypoxic of 8% oxygen was induced. Caspase-3 protein expression in renal tissue was assessed by immunohistochemical method. In the present study, the expressions of caspase-3 protein were significantly higher in saline-control hypoxic group than in morphine hypoxic preconditioning group ((29.3+/-5.7)% vs. (12.16+1.23)%, P<0.05). These observations suggested that morphine hypoxic preconditioning can protect rabbit against acute kidney hypoxic/reoxygenation injury by decreasing expression of caspase-3 protein.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Superóxido Dismutase
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Traumatismo por Reperfusão
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Distribuição Aleatória
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Precondicionamento Isquêmico
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Caspase 3
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Rim
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Metabolismo
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Métodos
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Morfina
Limite:
Animals
Idioma:
Zh
Revista:
J. biomed. eng
/
Sheng wu yi xue gong cheng xue za zhi
Ano de publicação:
2011
Tipo de documento:
Article