Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 385-390, 2008.
Article
em En
| WPRIM
| ID: wpr-359415
Biblioteca responsável:
WPRO
ABSTRACT
Two trace impurities in the bulk drug lisinopril were detected by means of high-performance liquid chromatography coupled with mass spectrometry (HPLC/MS) with a simple and sensitive method suitable for HPLC/MSn analysis. The fragmentation behavior of lisinopril and the impurities was investigated, and two unknown impurities were elucidated as 2-(6-amino-1-(1-carboxyethylamino)-1-oxohexan-2-ylamino)-4-phenylbutanoic acid and 6-amino-2-(1-carboxy-3-phenylpro-pylamino)-hexanoic acid on the basis of the multi-stage mass spectrometry and exact mass evidence. The proposed structures of the two unknown impurities were further confirmed by nuclear magnetic resonance (NMR) experiments after preparative isolation.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Espectroscopia de Ressonância Magnética
/
Contaminação de Medicamentos
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Cromatografia Líquida de Alta Pressão
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Lisinopril
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Espectrometria de Massas por Ionização por Electrospray
/
Métodos
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2008
Tipo de documento:
Article