Epigenetic Changes of Serotonin Transporter in the Patients with Alcohol Dependence: Methylation of an Serotonin Transporter Promoter CpG Island
Psychiatry Investigation
; : 130-133, 2011.
Article
em En
| WPRIM
| ID: wpr-35977
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: Psychiatric disorders such as depression, anxiety and alcohol dependence are associated with serotonin metabolism. We assessed the methylation level of the serotonin transporter (5-HTT) promoter region in control and alcohol dependent patients. METHODS: Twenty seven male patients who met the Diagnostic and Statistical Manual of Mental Disorder IV (DSM-IV) criteria for alcohol dependence were compared with fifteen controls. Polymerase chain reaction (PCR) assays of bisulfate-modified DNA were designed to amplify a part of the CpG island in the 5HTT gene. Pyrosequencing was performed and the methylation level at seven CpG island sites was measured. RESULTS: We found no differences in the methylation patterns of the serotonin transporter linked promoter region (5-HTTLPR) between alcohol-dependent and control subjects. CONCLUSION: Our negative finding may be because 5-HTT epigenetic variation may not affect the expression for 5-HTT or there may be other methylation site critical for its expression. To find out more conclusive result, repeating the study in more methylation sites with a larger number of samples in a well-controlled setting is needed.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Ansiedade
/
DNA
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Serotonina
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Reação em Cadeia da Polimerase
/
Regiões Promotoras Genéticas
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Ilhas de CpG
/
Depressão
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Alcoolismo
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Proteínas da Membrana Plasmática de Transporte de Serotonina
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Epigenômica
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Humans
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Male
Idioma:
En
Revista:
Psychiatry Investigation
Ano de publicação:
2011
Tipo de documento:
Article