Application of DNA chip techniques for Yq microdeletion analysis in infertile males
Experimental & Molecular Medicine
; : 179-184, 2004.
Article
em En
| WPRIM
| ID: wpr-37850
Biblioteca responsável:
WPRO
ABSTRACT
Our aim was to apply DNA chip technology as a diagnostic tool in infertility research and clinics. Six loci, including a sex-determining region on the Y chromosome and five sequence-tagged sites in azoospermia-factor regions were investigated in infertile male patients. Our method produced a sensitive signal, which showed the presence or absence of the STS regions on the Y chromosome. The results from 93 patients with non- obstructive azoospermia, oligoathenoteratozoospermia, or oligozoospermia were identical when analyzed with either the DNA chip technique or conventional PCR-gel electrophoresis. We have demonstrated its application in the molecular diagnosis of male infertility. This system provides an economic and high-throughput method for detecting the deletion of genomic DNA sequences of large groups of infertile patients, and a completely new approach to male infertility screening. The application of DNA chip technology to identify Yq deletions can also facilitate our understanding of male infertility.
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Base de dados:
WPRIM
Assunto principal:
Aberrações dos Cromossomos Sexuais
/
Análise Mutacional de DNA
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Reação em Cadeia da Polimerase
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Valor Preditivo dos Testes
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Sitios de Sequências Rotuladas
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Deleção Cromossômica
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Sensibilidade e Especificidade
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Análise de Sequência com Séries de Oligonucleotídeos
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Proteínas de Plasma Seminal
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Cromossomos Humanos Y
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Female
/
Humans
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Male
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2004
Tipo de documento:
Article