Effect of concentrations of sucrose on Streptococcus mutans gbpC expression / 中华微生物学和免疫学杂志

ABSTRACT

Objective To assess the influence of concentrations of sucrose on Streptococcus tautens (S. mutans) gbpC expression. Methods Total RNA was extracted from S. mutans after cultured in 0.5%, 1.0%, 5.0% sucrose and then reversely transcripted to cDNA. By TaqMan RT-RCR technique, we checked gbpC expression. Results Compared with 0.5% sucrose, 1.0% sucrose significantly upregulated gbpC expression(P<0.05); gbpC expression of more-adherent S. mutans strains was higher than that of less-adherent strains, Conclusion The upregulation of gbpC expression by sucrose may be one of the mechanisms underlying the increased adherence; There might be some correlation between gbpC expression andS. mutans adherence.