Co-circulation of Two Genotypes of Hepatitis A Virus from Sporadic Cases in Northeastern Area of Seoul, Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 371-377, 2008.
Article
em Ko
| WPRIM
| ID: wpr-39343
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: In previous studies, most hepatitis A virus (HAV) isolates had been genotype IA in Korea. Recently, a small number of different genotypes were reported with an upsurge of acute hepatitis by HAV. We investigated the distribution of HAV genotypes. METHODS: RNA was extracted from anti-HAV IgM positive sera which were collected from March 2007 to February 2008 at a tertiary care hospital in Northeastern Seoul, Korea. Nested reverse transcription (RT)-PCR and direct sequencing for VP1/P2A region of the HAV were performed. RESULTS: A total of 699 cases with suspected acute hepatitis were tested for anti-HAV IgM, and positive results were obtained in 56 sera (8.0%), which were collected 2 to 15 days (median, 7 days)after the onset of symptoms. Of the 56 seropositive samples, 52 (92.9%) were positive for HAV RNA, among which 28 isolates (53.8%) belonged to genotype IA and the remaining 24 (46.2%) belonged to genotype IIIA. Both IA and IIIA genotypes were isolated from 6-7 neighboring administrative districts throughout the year without geographic or seasonal restrictions. CONCLUSIONS: Co-circulation of two distinct HAV genotypes (IA and IIIA) was observed from the northeastern Seoul for the year studied.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Imunoglobulina M
/
RNA Viral
/
Dados de Sequência Molecular
/
Proteínas Estruturais Virais
/
Sequência de Aminoácidos
/
Análise de Sequência de DNA
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Vírus da Hepatite A Humana
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genótipo
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
Ko
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2008
Tipo de documento:
Article