DNT cells isolated and identified in the peripheral blood and its content detection / 国际外科学杂志
International Journal of Surgery
; (12): 154-157, 2009.
Article
em Zh
| WPRIM
| ID: wpr-395958
Biblioteca responsável:
WPRO
ABSTRACT
Objective The immune magnetic separation(MACS)method of DNT cells was set up and their content Was detected in peripheral blood of healthy man.Methods DNT cells were separated from the peripheral blood.Trypan blue staining and flow cytometry were used to detect their activity and purity,and flow cytometry Was used to detect their content in normal human peripheral blood.Results DNT cell activi ty using MACS sorting was>97%,the purity Was 82.77%;DNT cells accounted for(6.25±2.61)% (n=45)in the TCRαβ+T cells.Conclusion MACS Can quickly sort out high purity DNT cells,and do not affect the vitality of cells.DNT cells accounted for(6.25±2.61)%in the TCRCD3+T cells.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
International Journal of Surgery
Ano de publicação:
2009
Tipo de documento:
Article