Gold nanoparticles enhance anti-tumor effect of radiotherapy to hypoxic tumor
Radiation Oncology Journal
; : 230-238, 2016.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-39691
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Hypoxia can impair the therapeutic efficacy of radiotherapy (RT). Therefore, a new strategy is necessary for enhancing the response to RT. In this study, we investigated whether the combination of nanoparticles and RT is effective in eliminating the radioresistance of hypoxic tumors. MATERIALS ANDMETHODS:
Gold nanoparticles (GNPs) consisting of a silica core with a gold shell were used. CT26 colon cancer mouse model was developed to study whether the combination of RT and GNPs reduced hypoxia-induced radioresistance. Hypoxia inducible factor-1α (HIF-1α) was used as a hypoxia marker. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were conducted to evaluate cell death.RESULTS:
Hypoxic tumor cells had an impaired response to RT. GNPs combined with RT enhanced anti-tumor effect in hypoxic tumor compared with RT alone. The combination of GNPs and RT decreased tumor cell viability compare to RT alone in vitro. Under hypoxia, tumors treated with GNPs + RT showed a higher response than that shown by tumors treated with RT alone. When a reactive oxygen species (ROS) scavenger was added, the enhanced antitumor effect of GNPs + RT was diminished.CONCLUSION:
In the present study, hypoxic tumors treated with GNPs + RT showed favorable responses, which might be attributable to the ROS production induced by GNPs + RT. Taken together, GNPs combined with RT seems to be potential modality for enhancing the response to RT in hypoxic tumors.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Neoplasias Colorretais
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Radioterapia
/
Técnicas In Vitro
/
Sobrevivência Celular
/
Morte Celular
/
Espécies Reativas de Oxigênio
/
Neoplasias do Colo
/
Dióxido de Silício
/
DNA Nucleotidilexotransferase
/
Nanopartículas
/
Hipóxia
Limite:
Animais
Idioma:
Inglês
Revista:
Radiation Oncology Journal
Ano de publicação:
2016
Tipo de documento:
Artigo