Comparison of mouse lung fibrosis induced by intraperitoneal injection and intratracheal instillation of bleomycin / 中国免疫学杂志

ABSTRACT

Objective:To study the differences of bleomycin-induced pulmonary fibrosis in mice induced by intraperitoneal injection and intratracheal instillation of bleomycin.Methods:ICR mice (male,8 w of age,18 to 22 g bodyweight) were used.①ICR mice were randomly divided into three groups.In the group P,bleomycin was injected intraperitoneal five times in a dose of 40 mg/kg,and in the group I,bleomycin was instilled intratracheally in a dose of 5 mg/kg.The mice were killed at 14,28 or 40 day.②ICR mice were randomly divided into four groups bleomycin was injected intraperitoneal three,four or five times in a dose of 40 mg/kg,and in the group control ,bleomycin was injected intraperitoneal in a dose of 200 μl for five times.The mice was killed at 28 or 40 day.The pathological changes and symptoms were observed.Results:① The weight of the mice given blemycine were lost after injection.Symptoms were more serious in group P than those of group I.The pulmonary coefficient of group P was more than that of group I.At 28 day after injection,fibrosis was widely and stably formed mainly in around the bronchia and bronchioles,especially,near the pulmonary hilar area,in the group I mice,however,the same changes were mainly seen under the pleura or perivascular pulmonary tissues in the mice of group P.The pathological score of pulmonary fibrosis was different between those two groups.②Different dose of bleomycine induced different change of the mouse's weight.The most of all of the three group were five time injection; The lung index of five time injection of bleomycine was the most.The pulmonary fibrosis mouse model was made successfully.After comparising all of the data,we found intraperitoneal of bleomycine five times was the more convenient method.Conclusion:The sites of pulmonary fibrosis in the mice are different between the mice induced by intraperitoneal injection or intratracheal instillation of bleomycin.Intraperitoneal injection five times is a more convenient method to make pulmonary fibrosis.