Quantitative Determination of Saponins in the Root of Panax pseudo-ginseng var.notoginseng by HPLC-ELSD and UV Spectrophotometry / 中草药
Chinese Traditional and Herbal Drugs
; (24): 737-739, 2000.
Article
em Zh
| WPRIM
| ID: wpr-412227
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WPRO
ABSTRACT
A reverse phase HPLC-ELSD method for the determination of ginsenoside Rg1 in the rootof Panaa pseudo-ginseng var. notoginseng (Burkill) Hoo et Tseng was reported. Chromatographic condi-tions Shim-pack CLC-ODS column (6.0 mm×150 mm); acetonitrile-water (30 70) as the mobilephase; Shimadzu LC-6A with SEDEX-55 ELSD detector. The method was found to be simple and accuratewith recovery rate of 100. 50% and RSD= 1.82 %. The established UV spectrophotometric determinationof total saponins in P. pseudo-ginseng var. notoginseng was also tried and gave an accurate result coinci-dental with that of the HPLC results. The recovery rate was 101.50%, and RSD=1. 44%. It seemed thatboth methods can be used reliably for the quality control of P. pseudo-ginseng var. notoginseng.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2000
Tipo de documento:
Article